![]() ![]() Typical applications of DLS are particle size analysis, screening of buffer conditions during formulation, stability testing, and aggregation analysis. This protocol provides step-by-step instructions for preforming a basic DLS experiment assuming you have been trained on the instrument by Dr.Erbse or. It can thereby be applied to everything from antibody, peptides and proteins, to nanoparticles and viruses. Applicability:ĭynamic light scattering (DLS) is applicable to particles and macromolecules from ~ 1 nm to ~1 µm. The measured Brownian motion is related to the diffusion coefficient and from that the hydrodynamic size (Stokes radius) can be obtained. Dynamic light scattering (DLS) is an established method in the measuring range of nanometers and submicrometers. Principle:ĭynamic light scattering (DLS), also referred to as quasi-elastic light scattering (QELS) or photon correlation spectroscopy (PCS), is a spectroscopic technique that measures the Brownian motion of particles/macromolecules in solution. With high sensitivity it is ideally suited for detection of size changes even as a function of time. We use DLS instrumentation from Wyatt Technology and Malvern. Dynamic light scattering (DLS) is a robust, simple and non-contact method for the measurement of particle size and particle size distributions from the nanometre to the submicron range. Our instrumentation is temperature controlled which is handy for stability investigations. We have the traditional DLS cuvette format, but also a DLS plate reader for high-throughput analysis and screenings, that utilizes low sample volumes. Often these two techniques are combined in one instrument. ![]() A typical use of dynamic light scattering (DLS) at SOLVE is particle size analysis and screening of protein solutions for aggregates. Electrophoretic light scattering is based on dynamic light scattering. ![]()
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